tissue engineering and biomaterials laboratory facilities

The Tissue Engineering & Biomaterials Laboratory is part of the Fischell Department of Bioengineering at the University of Maryland.  The laboratory is housed in room 3237 of the new Jeong H. Kim Engineering Building, a state of the art engineering research center.  The laboratory consists of five dedicated spaces: laboratory space (800 ft2), a cell culture room (200 ft2), a microscopy and equipment room (100 ft2), a dissection room (100 ft2), and an environmental room with controlled temperature and humidity (100 ft2).  The lab is equipped for polymer synthesis, biomaterials fabrication and characterization, molecular biology studies, and cell biology studies.  In particular, the lab contains 4 chemical fume hoods (Safeaire), 2 biological safety cabinets (Forma Scientific), polymer synthesis equipment (reactors, vacuum pump, and mechanical mixer), a rotoevaporator (Buchi, R-205), a top loading balance (Ohaus, Voyager), an analytical balance (Ohaus, Analytical Plus), a 4°C refrigerator (Kenmore), a pH meter (Fisher), a mechanical testing device (Instron, Model 5565) containing load cells with capacity of 5 kN and 100 N, 2 CO2 incubators (Napco, 6000 and Thermo 8000WJ), a -20°C freezer (Kenmore), a phase contrast microscope (Zeiss, Axiostar Plus), an inverted microscope (Zeiss, Axiovert 40CFL), microscopic digital imaging system and image analysis software (Zeiss), a UV/Vis spectrophotometer (Genesys, 10uv), a microcentrifuge (Eppendorf, 5417C), a refrigerated microcentrifuge (Eppendorf, 5417R), a clinical centrifuge (Sorvall, Legend RT), electrophoresis equipment, a water filtration system (Millipore), a quantitative RT-PCR (Applied Biosystems, 7000), a high throughput quantitative RT-PCR (Applied Biosystems, 7900HT), a water bath (Fisher Scientific, Isotemp 210), a vortex (Scientific Industries), an oven (Fisher Scientific), tissue embedding center (Shandon), microtome (Leica, RM2155), a data analysis workstation (IBM), and 6 word processing workstations (Dell).  In addition, the Tissue Engineering & Biomaterials Laboratory is equipped with 1 work station for data analysis, 1 work station for image analysis, 5 word processing work stations, and an internal network assisted server (NetGear ReadyNAS, 2TB) for data storage as well as archive storage.  Finally, the Tissue Engineering & Biomaterials Laboratory has office space for 8 postdoctoral fellows / graduate students, in addition to separate office space for the PI.

fischell department of bioengineering facilities

The Tissue Engineering and Biomaterials Laboratory is also a primary member of the Core Bioengineering Laboratory within the Fischell Department of Bioengineering at the University of Maryland.  The Core Bioengineering Laboratory is a central laboratory dedicated the cell biology and molecular biology studies.  The Core Bioengineering Laboratory includes an autoclave (HotPack), a glass dishwasher (Heinicke, H-1125), 2 -80°C freezers (Revco), an ice maker, a nanopure diamond water purification system (Barnstead, 1370), a nanodrop spectrophotometer (ND1000), a gel documentation system (BioRad), a tissue processor (Leica, TP1020), a tissue embedder (Leica, EG1160), an oven (ThermoElectron), a bioanalyzer (Agilent, 2100), a microtome (Microm, HM355S), and a plate reader (Molecular Devices, SpectraMax M5).  The Core Bioengineering Laboratory has recently added a state of the art flow cytometry suite.  This suite contains two flow cytometers (Becton Dickenson, FACSCanto II and FACSAria II).  The FACSAria II is a three laser system that allows for quick and precise cell sorting.  The FACSCanto II is a 4/2 system that allows for high throughput analysis of the composition of heterogeneous cell populations.

functional macromolecular engineering laboratory facilities

The Tissue Engineering and Biomaterials Laboratory is also a primary member of the Functional Macromolecular Engineering Laboratory within the Fischell Department of Bioengineering at the University of Maryland.  The Functional Macromolecular Engineering Laboratory is dedicated to the analysis and characterization of macromolecular based materials, and is located in room 1213 of the Jeong H. Kim Engineering Building.  The 1000 ft² laboratory includes a differential scanning calorimeter (TA Instruments, Q100), a differential mechanical analyzer (TA Instruments, Q800), a dielectric analyzer (TA Instruments, 2970DEA), a gel permeation chromatography system (Waters), a refractive index detector (Waters, 2410), a light scattering detector (Wyatt, MiniDawn Tristar), a UV/Vis spectrophotometer (Perkin-Elmer, Lambda25), and an ultramicrotome system (Leica, U6).

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