Multiple Cloning Site of pUC18 and pUC19
Biochemical Engineering
The mcs (multiple cloning site) of pUC18 and pUC19 can be
recognized by many (about 3 dozens) restriction enzymes. The
recognition sites are identified below. The unique recognition
sites in pUC19 and the respective restriction enzymes are also
identified. The information below is obtained by running one of
the gene analysis programs (e.g., gene4.exe).
pUC18 DNA Sequence (base 230-289 of puc18.seq)
pUC19 DNA Sequence (base 230-289 of puc19.seq)
222222222222222222222222222222222222222222222222222222222222 pUC18 Base Number
888888888877777777776666666666555555555544444444443333333333 <----
987654321098765432109876543210987654321098765432109876543210
222222222222222222222222222222222222222222222222222222222222 pUC19 Base Number
333333333344444444445555555555666666666677777777778888888888 ---->
012345678901234567890123456789012345678901234567890123456789
cggttcgaacgtacggacgtccagctgagatctcctaggggcccatggctcgagcttaag pUC18 Sequence
gccaagcttgcatgcctgcaggtcgactctagaggatccccgggtaccgagctcgaattc pUC19 Sequence
--------------------------------------------------------------------------------------------
| | ag/ct Alu I
| ga/tc Dpn I
| c/cgg Hpa II, Msp I
| c/tag Mae I
| /gatc Mbo I, Nde II, Sau3A I
| gt/ac Rsa I
| | t/cga Taq I, TthHB8 I
| g/actc Hinf I*
| cc/cgg Nci I*
| cc/ggg Nci I*
| gt/cgac Acc I* (unique)
| g/gtacc Asp718 I (unique)
| c/ccggg Ava I* (unique)
| g/gatcc BamH I (unique)
| g/gtacc Ban I*
| gagct/c Ban II* (unique)
| gagct/c Bsp1286 I*
| g/gatcc Bst I (unique)
| g/aattc EcoR I (unique)
| gagct/c HgiA I*
| gtc/gac Hine II* (unique)
| gtc/gac Hind II* (unique)
| a/agctt Hind III (unique)
| ggtac/c Kpn I (unique)
| gga/tcc Nla IV*
| ggt/acc Nla IV*
| gagct/c Nsp II*
| c/ccggg Nsp III* (unique)
| ctgca/g Pst I (unique)
| gagct/c SacI (unique)
| g/tcgac Sal I (unique)
| ccc/ggg Sma I (unique)
| gcatg/c Sph I (unique)
| gagct/c Sst I (unique)
| t/ctaga Xba I (unique)
| c/ccggg Xcy I (unique)
| g/gatcc Xho II* (unique)
| c/ccggg Xma I (unique)
--------------------------------------------------------------------------------------------
cggttcgaacgtacggacgtccagctgagatctcctaggggcccatggctcgagcttaag pUC18 Sequence
gccaagcttgcatgcctgcaggtcgactctagaggatccccgggtaccgagctcgaattc pUC19 Sequence
222222222222222222222222222222222222222222222222222222222222 pUC18 Base Number
888888888877777777776666666666555555555544444444443333333333 <----
987654321098765432109876543210987654321098765432109876543210
222222222222222222222222222222222222222222222222222222222222 pUC19 Base Number
333333333344444444445555555555666666666677777777778888888888 ---->
012345678901234567890123456789012345678901234567890123456789
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Biochemical Engineering -- Multiple Cloning Site of pUC19
Forward comments to:
- Nam Sun Wang
- Department of Chemical & Biomolecular Engineering
- University of Maryland
- College Park, MD 20742-2111
- 301-405-1910 (voice)
- 301-314-9126 (FAX)
e-mail: nsw@umd.edu
©1996-2007 by Nam Sun Wang
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